Journal: Cancers

Article Title: Autocrine Signaling of NRP1 Ligand Galectin-1 Elicits Resistance to BRAF-Targeted Therapy in Melanoma Cells

doi: 10.3390/cancers12082218

Figure Lengend Snippet: Galectin-1 upregulation in melanoma samples and cell lines resistant to BRAF-targeted therapy. ( A ) The graph shows Galectin-1 (LGALS1 gene) mRNA expression variation in paired melanoma samples (obtained from seven patients, indicated by black diamonds) before treatment with BRAF inhibitors and after the onset of secondary resistance (Log2 ratio resistant/pretherapy), as well as in A375 and SK-MEL-28 melanoma cells before and after developing resistance to 2μM PLX-4720 (Log2 ratio Resistant/Parental; n = 3). ( B ) Analysis of Galectin-1 protein levels (by PikoKine ELISA assay) in conditioned media (CM) derived from either parental or PLX4720-resistant A375 and SK-MEL-28 (PLX-resistant) cells. Data are normalized to levels detected in the respective parental cells and represent the average of a technical duplicate. Statistical significance was assessed by t -test, comparing parental and their derived PLX-resistant cell lines; *** p < 0.0001.

Article Snippet: Galectin-1 concentrations in CM were measured by PikoKine ELISA assay (category EK0762; Boster Biological Technology, Pleasanton, CA, USA), according to the manufacturer’s protocol.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Derivative Assay

Journal: Frontiers in Aging Neuroscience

Article Title: Long-Term Administration of Triterpenoids From Ganoderma lucidum Mitigates Age-Associated Brain Physiological Decline via Regulating Sphingolipid Metabolism and Enhancing Autophagy in Mice

doi: 10.3389/fnagi.2021.628860

Figure Lengend Snippet: Improvement of multiple organ aging after long-term administration of TGL. TGL shows an improvement in brown adipose, liver, and kidney. (A) HE staining of brown adipose tissue; (B) Apoptosis rate of Liver using TUNEL staining; (C) TGL shows an improvement on aging in kidney using β-galactosidase staining. Data are presented as the means ± SD of more than six independent experiments. ** p < 0.01.

Article Snippet: Detection kit for β-galactosidase (β-Gal) activity , , Solarbio , BC2580 , http://www.solarbio.com/.

Techniques: Staining, TUNEL Assay

Journal: Frontiers in Aging Neuroscience

Article Title: Long-Term Administration of Triterpenoids From Ganoderma lucidum Mitigates Age-Associated Brain Physiological Decline via Regulating Sphingolipid Metabolism and Enhancing Autophagy in Mice

doi: 10.3389/fnagi.2021.628860

Figure Lengend Snippet:

Article Snippet: Detection kit for β-galactosidase (β-Gal) activity , , Solarbio , BC2580 , http://www.solarbio.com/.

Techniques: Recombinant, Activity Assay, TUNEL Assay, Staining, RNA Sequencing Assay

Journal: EXCLI Journal

Article Title: 5,6,7,4'-Tetramethoxyflavanone attenuates NADPH oxidase 1/4 and promotes sirtuin-1 to inhibit cell stress, senescence and apoptosis in Aß25-35–mediated SK-N-SH dysfunction

doi: 10.17179/excli2021-3841

Figure Lengend Snippet: TMF protects against cellular senescence in response to Aβ 25-35 toxicity in SK-N-SH cells via NOX1/4 and Sirt1. The cells were previously treated in the presence or absence of EX527 (1 µM) and GKT137831 (1 µM) for 1 hr, pretreated with TMF (10 µM) for 2 hr, and then treated with Aβ 25-35 (10 µM) for 24 hr. Western blot analysis was used to determine the expression of A. p53, B. p21, C. p-Rb/Rb, and D. Sirt1. E. SA-β-galactosidase assay was used to investigate the percentage of SA-β-galactosidase-positive cells in SK-N-SH cells. The values are shown as the mean ± SEM from 3 independent experiments (*** P ˂ 0.001, compared to the control treatment; # P ˂0.05, ## P ˂0.01, ### P ˂0.001, compared to Aβ 25-35 treatment alone. $ P ˂0.05, $$ P ˂0.01, $$$ P ˂0.001, compared to the individual group).

Article Snippet: Anti-β-actin, anti-lamin B1, anti-Bcl-x L , anti-Bax, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), 2′,7′-dichlorofluorescine diacetate, Muse® Mitopotential assay kit, Muse®Annexin V & Dead Cell kit, the Muse® Caspase-3/7 kit, and the BetaRed™ β-Gal assay kit were purchased from Merck Millipore (Millipore, MA, USA).

Techniques: Western Blot, Expressing